Automatic Real-Time Quantitative PCR: 6.1℃/s Ramp for Medical & Research Applications

Applicable supplies

0.2ml 96-well plate, 8 tubes, single tube(transparent, frosted, milky white are applicable)

Number of fluorescence channels

6

Applicable fluorescein

Channel 1: FAM, SYBR Green I, SYTO 9,EvaGreen, LC Green

Channel 2: HEX, VIC, TET, JOE

Channel 3: ROX, Texas Red

Channel 4: Cy5

Channel 5: Alexa Fluor 680

Channel 6: FRET

Applicable probe

Taqman probe, molecular beacon probe,scorpion probe, FRET probe, etc.

Reaction system

0～100μl

Linear range

1～1010 copies

Sample detection repeatability

Ct value CV≤0.5%

Sample linear

/r/≥0.999

Control method

Stand-alone operation: Use the instrument’s10.4-inch touch screen and software system to create a new experiment and runit.

Network operation:

(1) DirectPC connection: After the instrument is connected to the PC via apoint-to-point network, the application software on the computer is used toimplement experiments, monitoring, data analysis and other operations;

(2) LANaccess: By setting the network parameters of the instrument, the instrumentcan be connected to the local LAN, so that any computer in the local areanetwork can perform operations such as instrument monitoring, datasynchronization, and analysis.

Automatic sample bin

Sample compartment can be automaticallypopped / closed by touch screen control;

When the pop-up state is closed, you cantouch the sample compartment to close automatically.

Power failure protection

With thefunction of automatically resuming the experiment when the power is cut offand then power on, there is no need to wait for the PC and software to open

Data transmission

Experimentaldata can be imported and exported via USB flash drive

Storage

Can store more than 1000 experiment datafiles

Optical system

Light source

Bright long-life maintenance-free LED lightsource

Detector

Photodiode (PD)

Detection location

Top excitation, top scan

Detection method

Six fluorescence channels are scanned fromhole to hole simultaneously without fluorescence edge effect

Detection time

Complete detection of 96 wells in 6fluorescence channels in 7 seconds

Excitation light wavelength

Channel 1: 465nm Channel 2: 527nm Channel 3:580nm Channel 4: 632nm Channel 5: 680nm Channel 6: 465nm

Detection light wavelength

Channel 1: 510nm Channel 2: 563nm Channel 3: 616nmChannel 4: 664nm Channel 5: 730nm Channel 6: 616nm

Fluorescence linear

r≥0.990

Dynamic range for fluorescence detection

Dynamic range of fluorescence detection can be adjustedaccording to reagents

Temperature Control System

Cover temperature

Room temperature～110℃

Constant pressure hot cap

Compatible with consumables of differentheights

Module temperature

0℃～100℃

Module temperature control principle

Peltier effect, semiconductor refrigerationtechnology

Maximum temperature rise / fall rate

Maximum heating rate ≥6.1℃/ s Maximum cooling rate ≥5.0℃/ s

Temperature uniformity

±0.1℃

Temperature accuracy

≤±0.1℃

Gradient temperature width

1℃～40℃

Gradient temperature number

12 columns

Special temperature setting function

Supports thermal gradient PCR experiments,Long PCR, Touch Down PCR

Software functions

Analysis function

Qualitative analysis, absolute quantitativeanalysis, relative quantitative analysis, endpoint fluorescence analysis,melting curve analysis, SNP analysis, etc.

Report customization

Pre-stored multiple industry experimentreport models;

Board full open universal report function,users can customize report content and form;

Rights management function

The administrator account can limit the functions of“manually set thresholds”, “running settings”, “running experiments”, and“analysis data” of ordinary accounts to manage the use rights of instrumentsof ordinary accounts

Resource sharing function

After the instrument is connected to the PC,both parties can realize the synchronous sharing of experimental data throughoperations such as uploading and downloading.